Experiments in Biochemistry: A Hands-on Approach, 2nd Edition

Shawn O. Farrell, Lynn E. Taylor

Copyright 2006
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Experiments in Biochemistry: A Hands-on Approach 2nd Edition by Shawn O. Farrell/Lynn E. Taylor

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Overview
Meet the Authors
What's New
Features
Table of Contents
Pricing

Overview

EXPERIMENTS IN BIOCHEMISTRY: A HANDS-ON APPROACH, Second Edition features a variety of hands-on, classroom tested experiments that are proven to work and can be completed in a normal lab period. The manual's stand-alone experiments are effective in courses meeting only once a week, giving students a broad overview of the subject matter. A more comprehensive set of experiments is also available and allows students to delve further into each of the topics presented. The Second Edition also features new and revised experiments, including a new experiment that involves cloning the barracuda LDH gene! Students and professors will also find expanded problem sets in this edition. "Tip" boxes, located throughout the text, provide pointers to students on how to perform the experiment at hand, while "Essential Information" boxes highlight pertinent information that will help the student complete the experiment. The second edition continues to include references and further readings at the end of each chapter.

Meet the Authors

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Shawn O. Farrell

Shawn O. Farrell, a native of Northern California, received his B.S. in biochemistry from University of California, Davis, studying carbohydrate metabolism. He completed his Ph.D. in biochemistry at Michigan State University, where he focused on the study of fatty acid metabolism. Dr. Farrell became interested in biochemistry while in college, as it was relevant to his passion for bicycle racing. He raced competitively for 15 years and now officiates bicycle races worldwide. He has taught biochemistry lecture and laboratory courses at Colorado State University for 16 years and now works for USCycling. Professor Farrell has written scientific journal articles about specific research projects and about laboratory teaching, as well as articles for sports publications, such as "Salmon, Trout, and Steelheader" magazine. He is co-author with Mary Campbell on BIOCHEMISTRY, 7e (Cengage Learning).

Lynn E. Taylor

Lynn Taylor grew up in Minnesota and received her B.A. in Genetics from UC Berkeley. As a research assistant, she studied galactose oxidation in liver at Michigan State University, and cyctochrome c oxidase expression in yeast at the University of Colorado-Boulder. Ms. Taylor's interest in molecular biology led her to Colorado State University, where she obtained her M.S. in Biochemistry and Molecular Biology. She has spent 15 years investigating the regulation and expression of key regulatory enzymes involved in maintaining acid-base balance in the kidney, and currently holds the position of senior research associate.

What's New

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In Experiment 12, students will clone the barracuda LDH gene into an expression vector, making a HIS-Tagged fusion protein that can be purified by affinity chromatography. Students will create a new vector for doing expression that makes the ligation of DNA easier and more efficient while learning about expanded methods for purification of HIS-Tagged Lactate Dehydrogenase via Affinity Chromatography.
The Second Edition includes expanded problem sets for further biochemistry practice and study.

Features

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All experiments have been classroom tested through multiple semesters and can be completed in a normal lab period.
Alternate versions of experiments allow for easy use in labs which meet only once a week or multiple times in a week.
"Tip" boxes throughout text give students pointers on how to perform the labs.
"Essential Information" boxes highlight pertinent information.

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Introduction to the Text.
Objectives of the Biochemistry Laboratory.
Chapter Format of Hands on Biochemistry.
1. Biochemistry Boot Camp.
2. Acids, Bases, and Buffers.
3. Spectrophotometry.
4. Enzyme Purification.
5. Ion Exchange Chromatography.
6. Affinity Chromatography.
7. Gel filtration Chromatography.
8. Enzyme Kinetics.
9. Electrophoresis.
10. Western Blots.
11. Restriction Enzymes.
12. Cloning and Expression of Foreign Proteins.
13. Polymerase Chain Reaction.